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Nat Methods. 2010 Jul;7(7):535-40. doi: 10.1038/nmeth.1468. Epub 2010 Jun 6.

Two-photon calcium imaging from head-fixed Drosophila during optomotor walking behavior.

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1
Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.

Erratum in

  • Nat Methods. 2011 Feb;8(2):184.

Abstract

Drosophila melanogaster is a model organism rich in genetic tools to manipulate and identify neural circuits involved in specific behaviors. Here we present a technique for two-photon calcium imaging in the central brain of head-fixed Drosophila walking on an air-supported ball. The ball's motion is tracked at high resolution and can be treated as a proxy for the fly's own movements. We used the genetically encoded calcium sensor, GCaMP3.0, to record from important elements of the motion-processing pathway, the horizontal-system lobula plate tangential cells (LPTCs) in the fly optic lobe. We presented motion stimuli to the tethered fly and found that calcium transients in horizontal-system neurons correlated with robust optomotor behavior during walking. Our technique allows both behavior and physiology in identified neurons to be monitored in a genetic model organism with an extensive repertoire of walking behaviors.

PMID:
20526346
PMCID:
PMC2945246
DOI:
10.1038/nmeth.1468
[Indexed for MEDLINE]
Free PMC Article
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