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Future Microbiol. 2010 Jun;5(6):971-80. doi: 10.2217/fmb.10.49.

Epidemic of surgical-site infections by a single clone of rapidly growing mycobacteria in Brazil.

Author information

1
Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, Rua Botucatu, 862 3 degrees Andar, 04023-062 - São Paulo, SP, Brazil. sylvia.leao@unifesp.br

Abstract

AIM:

Our aim is to investigate if the clusters of postsurgical mycobacterial infections, reported between 2004 and 2008 in seven geographically distant states in Brazil, were caused by a single mycobacterial strain.

MATERIALS & METHODS:

Available information from 929 surgical patients was obtained from local health authorities. A total of 152 isolates from surgical patients were identified by PCR restriction enzyme analysis of the hsp65 gene (PRA-hsp65) and sequencing of the rpoB gene. Isolates were typed by pulsed-field gel electrophoresis (PFGE) using two restriction enzymes, DraI and AseI. A total of 15 isolates not related to surgical cases were analyzed for comparison.

RESULTS:

All isolates were identified as Mycobacterium abscessus ssp. massiliense. Isolates from surgical patients and one sputum isolate grouped in a single PFGE cluster, composed of two closely related patterns, with one band difference. A total of 14 other isolates unrelated to surgical cases showed distinctive PFGE patterns.

CONCLUSION:

A particular strain of M. abscessus ssp. massiliense was associated with a prolonged epidemic of postsurgical infections in seven Brazilian states, suggesting that this strain may be distributed in Brazilian territory and better adapted to cause surgical-site infections.

PMID:
20521940
DOI:
10.2217/fmb.10.49
[Indexed for MEDLINE]
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