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Curr Protoc Cell Biol. 2010 Jun;Chapter 3:Unit3.36. doi: 10.1002/0471143030.cb0336s47.

Isolation of nucleoli.

Author information

1
Université de Lyon, Lyon, France.

Abstract

Nucleoli are now recognized as multi-functional nuclear domains involved in several fundamental cell processes such as ribosome biogenesis, regulation of the assembly of non-ribosomal ribonucleoprotein complexes, tRNA maturation, sequestration of protein, viral infection, and cellular ageing. Extensive proteomic analyses of these nucleolar domains after their purification have contributed to the description of their multiple biological functions. Because nucleoli are the largest and densest nuclear structures, they are easily amenable to purification from nuclei of cultured animal cells using the protocol described in this unit.

PMID:
20521233
DOI:
10.1002/0471143030.cb0336s47
[Indexed for MEDLINE]

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