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J Virol. 2010 Aug;84(16):8353-8. doi: 10.1128/JVI.00696-10. Epub 2010 Jun 2.

Influence of pr-M cleavage on the heterogeneity of extracellular dengue virus particles.

Author information

1
Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.

Abstract

During dengue virus replication, an incomplete cleavage of the envelope glycoprotein prM, generates a mixture of mature (prM-less) and prM-containing, immature extracellular particles. In this study, sequential immunoprecipitation and cryoelectron microscopy revealed a third type of extracellular particles, the partially mature particles, as the major prM-containing particles in a dengue serotype 2 virus. Changes in the proportion of viral particles in the pr-M junction mutants exhibiting altered levels of prM cleavage suggest that the partially mature particles may represent an intermediate subpopulation in the virus maturation pathway. These findings are consistent with a model suggesting the progressive mode of prM cleavage.

PMID:
20519400
PMCID:
PMC2916513
DOI:
10.1128/JVI.00696-10
[Indexed for MEDLINE]
Free PMC Article
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