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Clin Biochem. 2010 Aug;43(12):948-56. doi: 10.1016/j.clinbiochem.2010.04.075. Epub 2010 May 16.

Perturbations in the lipid profile of individuals with newly diagnosed type 1 diabetes mellitus: lipidomics analysis of a Diabetes Antibody Standardization Program sample subset.

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1
Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99352, USA.

Abstract

OBJECTIVES:

To characterize the lipid profile of individuals with newly diagnosed type 1 diabetes mellitus using LC-MS-based lipidomics and the accurate mass and time (AMT) tag approach.

DESIGN AND METHODS:

Lipids were extracted from plasma and sera of 10 subjects from the Diabetes Antibody Standardization Program (years 2000-2005) and 10 non-diabetic subjects and analyzed by capillary liquid chromatography coupled with a hybrid ion-trap-Fourier transform ion cyclotron resonance mass spectrometer. Lipids were identified and quantified using the AMT tag approach.

RESULTS:

Five hundred fifty-nine lipid features differentiated (q<0.05) diabetic from healthy individuals in a partial least-squares analysis, characterizing individuals with recently diagnosed type 1 diabetes mellitus.

CONCLUSIONS:

A lipid profile associated with newly diagnosed type 1 diabetes may aid in further characterization of biochemical pathways involved in lipid regulation or mobilization.

PMID:
20519132
PMCID:
PMC2919299
DOI:
10.1016/j.clinbiochem.2010.04.075
[Indexed for MEDLINE]
Free PMC Article
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