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Nat Biotechnol. 2010 Jun;28(6):581-3. doi: 10.1038/nbt.1631. Epub 2010 May 30.

Synthetic polymer coatings for long-term growth of human embryonic stem cells.

Author information

1
Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, Michigan, USA.

Abstract

We report a fully defined synthetic polymer coating, poly[2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl)ammonium hydroxide] (PMEDSAH), which sustains long-term human embryonic stem (hES) cell growth in several different culture media, including commercially available defined media. The development of a standardized, controllable and sustainable culture matrix for hES cells is an essential step in elucidating mechanisms that control hES cell behavior and in optimizing conditions for biomedical applications of hES cells.

PMID:
20512122
PMCID:
PMC3471651
DOI:
10.1038/nbt.1631
[Indexed for MEDLINE]
Free PMC Article

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