Format

Send to

Choose Destination
See comment in PubMed Commons below
Appl Environ Microbiol. 2010 Jul;76(14):4914-7. doi: 10.1128/AEM.00958-10. Epub 2010 May 28.

Engineering of Clostridium phytofermentans Endoglucanase Cel5A for improved thermostability.

Author information

1
Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA.

Abstract

A family 5 glycoside hydrolase from Clostridium phytofermentans was cloned and engineered through a cellulase cell surface display system in Escherichia coli. The presence of cell surface anchoring, a cellulose binding module, or a His tag greatly influenced the activities of wild-type and mutant enzymes on soluble and solid cellulosic substrates, suggesting the high complexity of cellulase engineering. The best mutant had 92%, 36%, and 46% longer half-lives at 60 degrees C on carboxymethyl cellulose, regenerated amorphous cellulose, and Avicel, respectively.

PMID:
20511418
PMCID:
PMC2901741
DOI:
10.1128/AEM.00958-10
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Support Center