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Vaccine. 2010 May 26;28 Suppl 2:B32-7. doi: 10.1016/j.vaccine.2009.10.154.

Dynamics of memory and naïve CD8+ T lymphocytes in humanized NOD/SCID/IL-2Rgammanull mice infected with CCR5-tropic HIV-1.

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Laboratory of Viral Pathogenesis, Institute for Virus Research, Kyoto University, Kyoto 606-8507, Japan.


Creating a novel small animal model of HIV-1 infection that can support long-term systemic HIV-1 infection and produce HIV-1-specific immune response has a great benefit for studying HIV-1 pathogenesis in vivo. In the present study, we have generated a humanized mouse, NOG-hCD34 mouse, by transplanting newborn NOD/SCID/IL-2Rgamma(null) mice with human hematopoietic stem cells through hepatic injection. These mice were infected with a CCR5-tropic HIV-1 and were analyzed for plasma viral load, changes in peripheral blood T lymphocytes, and HIV-1-specific antibody production. High level of viral replication, increase in effector/memory CD8(+) T lymphocytes, class-switching to IgG, and production of HIV-1-specific IgGs were observed. Our findings suggest that NOG-hCD34 mice may have a wide variety of application in HIV-1 research.

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