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Biochemistry. 2010 Jun 29;49(25):5200-5. doi: 10.1021/bi100412v.

Structural analysis of botulinum neurotoxin type G receptor binding .

Author information

1
Department of Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA.

Abstract

Botulinum neurotoxin (BoNT) binds peripheral neurons at the neuromuscular junction through a dual-receptor mechanism that includes interactions with ganglioside and protein receptors. The receptor identities vary depending on BoNT serotype (A-G). BoNT/B and BoNT/G bind the luminal domains of synaptotagmin I and II, homologous synaptic vesicle proteins. We observe conditions under which BoNT/B binds both Syt isoforms, but BoNT/G binds only SytI. Both serotypes bind ganglioside G(T1b). The BoNT/G receptor-binding domain crystal structure provides a context for examining these binding interactions and a platform for understanding the physiological relevance of different Syt receptor isoforms in vivo.

PMID:
20507178
PMCID:
PMC2894633
DOI:
10.1021/bi100412v
[Indexed for MEDLINE]
Free PMC Article

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