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J Sep Sci. 2010 Jul;33(13):1897-903. doi: 10.1002/jssc.200900846.

Development and validation of an improved reversed-phase liquid chromatographic method combined with pulsed electrochemical detection for the analysis of netilmicin.

Author information

1
Laboratorium voor Farmaceutische Analyse, Faculteit Farmaceutische Wetenschappen, Katholieke Universiteit Leuven, Leuven, Belgium.

Abstract

Netilmicin is one of the aminoglycoside antibiotics that lacks a strong UV absorbing chromophore. However, the application of pulsed electrochemical detection has been used successfully for the direct analysis of aminoglycoside antibiotics. This study describes an improved LC method combined with pulsed electrochemical detection for the analysis of netilmicin. Using a Zorbax SB C-18 column (250 mm x 4.6 mm id, 5 microm), isocratic elution was carried out with a mobile phase containing sodium sulfate (20 g/L), sodium octanesulfonate (0.3 g/L), THF (20 mL/L), and 0.2 M phosphate buffer pH 3.0 (50.0 mL/L). The robustness of the method was examined by means of an experimental design. The method proved to be sensitive, repeatable, linear, and robust. The method has also been used to analyze some commercial netilmicin samples.

PMID:
20506425
DOI:
10.1002/jssc.200900846
[Indexed for MEDLINE]

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