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Biotechnol Bioeng. 2010 Aug 1;106(5):699-706. doi: 10.1002/bit.22775.

Recombinant human cytochrome P450 monooxygenases for drug metabolite synthesis.

Author information

1
Novartis Institutes for Biomedical Research, GDC/PSB, WSJ-508.2.02A, CH-4056 Basel, Switzerland.

Abstract

Cytochrome P450 monooxygenases (CYPs) are important enzymes in the metabolism of xenobiotics. Therefore, several approaches to clone and overexpress the human isoforms have been made. In addition to microsomes or S9 preparations, these recombinant human isoforms have found diverse application in drug development. We discuss and give examples of the use of bacterial whole cell systems with rec. human CYPs for the preparative scale synthesis of drug metabolites.

PMID:
20506361
DOI:
10.1002/bit.22775
[Indexed for MEDLINE]

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