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J Am Chem Soc. 2010 Jun 23;132(24):8244-5. doi: 10.1021/ja102257v.

Visible fluorescence detection of type III protein secretion from bacterial pathogens.

Author information

1
Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, New York, New York 10065, USA.

Abstract

Type III protein secretion is essential for many gram-negative bacterial infections of host cells and an attractive target for new antibacterial drugs. Here, we describe a bacterial protein effector-carboxypeptidase G2 (CPG2) reporter system for fluorescence and visible detection of type III protein secretion in Salmonella typhimurium. This system provides a general method for measuring protein expression and secretion as well as a high-throughput and quantitative assay for analyzing type III protein secretion inhibitors.

PMID:
20504019
PMCID:
PMC2919334
DOI:
10.1021/ja102257v
[Indexed for MEDLINE]
Free PMC Article

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