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Eur J Clin Nutr. 2010 Jul;64(7):678-84. doi: 10.1038/ejcn.2010.92. Epub 2010 May 26.

Influence of the type of indigestible carbohydrate on plasma and urine short-chain fatty acid profiles in healthy human volunteers.

Author information

1
Department of Gastrointestinal Research and Leuven Food and Nutrition Research Centre (LFoRCe), Katholieke Universiteit Leuven, Leuven, Belgium. kristin.verbeke@uz.kuleuven.be

Abstract

BACKGROUND/OBJECTIVES:

Health effects of whole grain foods are becoming more evident. In this study, we analysed the short-chain fatty acid profiles in urine and serum derived from the colonic fermentation process of (13)C-barley meals, prepared from barley grown under (13)CO(2) atmosphere.

SUBJECTS/METHODS:

In a crossover study, five volunteers ingested intact barley kernels (high content of non-starch polysaccharides (NSP) and resistant starch (RS)) and barley porridge (high content of NSP only). Using a newly developed stable isotope technology, we monitored 14 and 24 h postprandially (13)C-acetate, (13)C-propionate and (13)C-butyrate in plasma and urine, respectively. The oro-cecal transit time (OCTT) of the meals was measured with the hydrogen breath test.

RESULTS:

The OCTT was 6 h and did not differ between the two test meals. An increase of (13)C-acetate was observed already early after ingestion of the meals (<6 h) and was attributed to early fermentation of the test meal. A rise in plasma (13)C-propionate in the fermentation phase could only be detected after the porridge and not after the kernel meal. An increase in (13)C-butyrate was only found in the fermentation phase and was higher after the barley kernels. Urine (13)C-short-chain fatty acids data were consistent with these observations.

CONCLUSIONS:

The difference in the profiles of (13)C-acetate, (13)C-propionate and (13)C-butyrate indicates that NSP combined with RS results in an altered fermentation profile than dietary fibre alone.

PMID:
20502475
DOI:
10.1038/ejcn.2010.92
[Indexed for MEDLINE]

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