Format

Send to

Choose Destination
See comment in PubMed Commons below
Obstet Gynecol. 2010 Jun;115(6):1209-16. doi: 10.1097/AOG.0b013e3181e01415.

Rapid polymerase chain reaction assay to detect herpes simplex virus in the genital tract of women in labor.

Author information

1
Department of Obstetrics and Gynecology, University of Washington, Seattle, Washington 98195, USA. cgardel@u.washington.edu

Abstract

OBJECTIVE:

To develop a rapid quantitative real-time polymerase chain reaction (PCR) to detect herpes simplex virus (HSV) in the genital secretions of women that may be used in labor.

METHODS:

Samples of genital secretions from women in labor, swabs of active genital lesions, and swabs of buffer solution were analyzed using a newly developed rapid HSV PCR assay to detect HSV glycoprotein B gene and quantitate virion copy number. A previously validated TaqMan PCR to detect HSV glycoprotein B gene was performed as the comparator gold standard. Positivity determination that optimized sensitivity and specificity was determined with receiver operating characteristic curves.

RESULTS:

The median time to result for rapid HSV PCR was 2 hours (range 1.5-3.5 hours). A positivity determination rule that required both wells of the rapid test to detect 150 copies or greater of HSV per milliliter maximized specificity (96.7%) without appreciable loss of sensitivity (99.6%). Among positive samples, the correlation between the rapid test and TaqMan for the quantity of HSV isolated was excellent (R=0.96, P<.001). The rapid test had a positive predictive value of 96.7% and a negative predictive value of 99.6% in a population with HSV shedding prevalence of 10.8%, based on the prevalence of genital HSV previously found among HSV-2 seropositive women in labor.

CONCLUSION:

Rapid HSV PCR provides results with excellent sensitivity and specificity within a timeframe that could inform clinical decision making for identifying neonates at risk of neonatal HSV infection.

LEVEL OF EVIDENCE:

II.

PMID:
20502292
PMCID:
PMC3034453
DOI:
10.1097/AOG.0b013e3181e01415
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Wolters Kluwer Icon for PubMed Central
    Loading ...
    Support Center