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Biomarkers. 2010 Sep;15(6):488-97. doi: 10.3109/1354750X.2010.485700.

Exposure to naphthalene induces naphthyl-keratin adducts in human epidermis in vitro and in vivo.

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1
Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Abstract

We observed naphthyl-keratin adducts and dose-related metabolic enzyme induction at the mRNA level in reconstructed human epidermis in vitro after exposure to naphthalene. Immunofluorescence detection of 2-naphthyl-keratin-1 adducts confirmed the metabolism of naphthalene and adduction of keratin. We also observed naphthyl-keratin adducts in dermal tape-strip samples collected from naphthalene-exposed workers at levels ranging from 0.004 to 6.104 pmol adduct microg(-1) keratin. We have demonstrated the ability of the human skin to metabolize naphthalene and to form naphthyl-keratin adducts both in vitro and in vivo. The results indicate the potential use of keratin adducts as biomarkers of dermal exposure.

PMID:
20500019
PMCID:
PMC2923669
DOI:
10.3109/1354750X.2010.485700
[Indexed for MEDLINE]
Free PMC Article

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