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Antimicrob Agents Chemother. 2010 Aug;54(8):3509-11. doi: 10.1128/AAC.00485-10. Epub 2010 May 24.

High-resolution melt curve analysis for identification of single nucleotide mutations in the quinolone resistance gene aac(6')-Ib-cr.

Author information

1
Department of Clinical Microbiology and Infectious Diseases, Hadassah Ein-Kerem, Jerusalem 91120, Israel. carloshg@hadassah.org.il

Abstract

We have developed a simple PCR-based high-resolution melt curve analysis for identification of the quinolone resistance gene aac(6')-Ib-cr through regions encompassing the two defining single nucleotide mutations. Dissociation curves showed 100% concordance with DNA sequencing, including the identification of a strain where aac(6')-Ib and aac(6')-Ib-cr coexist.

PMID:
20498320
PMCID:
PMC2916293
DOI:
10.1128/AAC.00485-10
[Indexed for MEDLINE]
Free PMC Article
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