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Eye (Lond). 2010 Sep;24(9):1503-8. doi: 10.1038/eye.2010.66. Epub 2010 May 21.

Effect of pupil dilation on retinal nerve fibre layer thickness measurements and their repeatability with Cirrus HD-OCT.

Author information

1
GB Bietti Eye Foundation - IRCCS, Rome, Italy. giacomo.savini@alice.it

Abstract

PURPOSE:

To assess whether pupil dilation influences retinal nerve fibre layer (RNFL) thickness measurements provided by spectral-domain optical coherence tomography (SD-OCT) in healthy individuals.

PATIENTS AND METHODS:

In this observational case series, carried out in a private clinical practice, 32 eyes of 32 participants were investigated. Using Cirrus HD-OCT (Carl Zeiss Meditec, Dublin, CA, USA) three individual 200 × 200 cube optic disc scans were obtained before and after pupil dilation. The RNFL thickness was the outcome measure. Coefficient of variation (COV) and test-retest variability were calculated.

RESULTS:

Pupil size did not influence RNFL thickness measurements: mean values did not change in any sector (except the 9 o'clock hour) after dilation. Excellent repeatability was achieved both before and after mydriasis. In the former condition, COV ranged between 1.37% (for average RNFL) and 4.46% (for clock hour 2 RNFL) and test-retest variability between 2.17 (for temporal quadrant RNFL) and 9.18 microm (for clock hour 6 RNFL). In the latter condition, COV ranged between 1.36% (for average RNFL) and 4.48% (for clock hour 2 RNFL) and test-retest variability between 2.41 (for average RNFL) and 9.29 microm (for clock hour 6 RNFL). The repeatability was higher than that previously reported for time-domain OCT.

CONCLUSION:

In eyes with clear media highly repeatable measurements of the RNFL thickness can be obtained by SD-OCT both before and after mydriasis.

PMID:
20489736
DOI:
10.1038/eye.2010.66
[Indexed for MEDLINE]

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