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Hum Exp Toxicol. 2011 Mar;30(3):192-8. doi: 10.1177/0960327110371696. Epub 2010 May 20.

Differential protection of pre-, co- and post-treatment of curcumin against hydrogen peroxide in PC12 cells.

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1
Indian Institute of Toxicology Research, Lucknow, India (Council of Scientific & Industrial Research, New Delhi, India.

Abstract

Pharmacological potential of curcumin was assessed in PC12 cells against hydrogen peroxide (H(2) O(2)) exposure. In MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] and lactate dehydrogenase (LDH) assays, 24-hour exposure of H(2)O(2) (0.5 mM and above) was found to be cytotoxic. A significant (p < 0.001) increase in percentage cell viability was recorded in PC12 cells pretreated with curcumin (25, 50 and 100 µg/mL) for 24 hours prior to H(2)O(2) (0.5 and 1 mM) exposure for 24 hours. Co-exposure to H(2)O(2) and curcumin was also found effective. However, a therapeutic treatment of curcumin for 24 hours after H(2)O(2) exposure to the cells was found ineffective. Differential response of PC12-H(2)O(2) model to curcumin in MTT and LDH assays suggests the utility of these endpoints to sort the drug candidates to study their antioxidant potential.

PMID:
20488840
DOI:
10.1177/0960327110371696
[Indexed for MEDLINE]

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