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FEBS Lett. 2010 Jul 2;584(13):2791-5. doi: 10.1016/j.febslet.2010.05.013. Epub 2010 May 17.

The archaeal exosome localizes to the membrane.

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Institut für Mikrobiologie und Molekularbiologie der JLU Giessen, Giessen, Germany.


We studied the cellular localization of the archaeal exosome, an RNA-processing protein complex containing orthologs of the eukaryotic proteins Rrp41, Rrp42, Rrp4 and Csl4, and an archaea-specific subunit annotated as DnaG. Fractionation of cell-free extracts of Sulfolobus solfataricus in sucrose density gradients revealed that DnaG and the active-site comprising subunit Rrp41 are enriched together with surface layer proteins in a yellow colored ring, implicating that the exosome is membrane-bound. In accordance with this assumption, DnaG and Rrp41 were detected at the periphery of the cell by immunofluorescence microscopy. Our finding suggests that RNA processing in Archaea is spatially organized.

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