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Macromol Biosci. 2010 Jul 7;10(7):775-82. doi: 10.1002/mabi.200900481.

Characterization of reversible associations by sedimentation velocity with UltraScan.

Author information

1
Department of Biochemistry, The University of Texas Health Science Center at San Antonio, San Antonio, Texas 78229, USA. demeler@biochem.uthscsa.edu

Abstract

We compare here the utility of sedimentation velocity (SV) to sedimentation equilibrium (SE) analysis for the characterization of reversible systems. Genetic algorithm optimization in UltraScan is used to optimize the model and to obtain solution properties of all components present in the system. We apply our method to synthetic and experimental data, and suggest limits for the accessible kinetic range. We conclude that equilibrium constants obtained from SV and SE analysis are equivalent, but that SV experiments provide better confidence for the K(d), can better account for the presence of contaminants and provide additional information including rate constants and shape parameters.

PMID:
20486142
DOI:
10.1002/mabi.200900481
[Indexed for MEDLINE]

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