Format

Send to

Choose Destination
Nat Biotechnol. 2010 Jun;28(6):617-623. doi: 10.1038/nbt.1628. Epub 2010 May 16.

Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Author information

1
Integrative Omics Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon, Korea.
2
Cancer Research UK, The London Research Institute, 44, Lincoln's Inn Fields, LondonWC2A 3PX, UK.
3
Department of Bio and Brain Engineering, Korea Advanced Institute of Science & Technology (KAIST), Yuseong, Daejeon, Korea.
4
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1HH, UK.
5
Bioneer Corporation, Daedeok, Daejeon, Korea.
6
Bioevaluation Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Ochang, Chungcheongbuk-do, Korea.
7
Department of Bioinformatics & Biotechnology, Korea University, Jochiwon, Chungnam, Korea.
8
School of Biological Sciences, Seoul National University, Seoul, Korea.
9
Department of Microbiology, Chungnam National University, Yuseong, Daejeon, Korea.
10
Division of Life Sciences, Kangwon National University, Chuncheon, Kangwon-do, Korea.
11
Department of Biochemistry, Yonsei University, Seoul, Korea.
12
Department of Food and Nutrition, Chungnam National University, Yuseong, Daejeon, Korea.
13
The Rockefeller University, 1230 York Avenue, New York, NY 10021-6399, USA.
#
Contributed equally

Erratum in

  • Nat Biotechnol. 2010 Dec;28(12):1308.

Abstract

We report the construction and analysis of 4,836 heterozygous diploid deletion mutants covering 98.4% of the fission yeast genome providing a tool for studying eukaryotic biology. Comprehensive gene dispensability comparisons with budding yeast--the only other eukaryote for which a comprehensive knockout library exists--revealed that 83% of single-copy orthologs in the two yeasts had conserved dispensability. Gene dispensability differed for certain pathways between the two yeasts, including mitochondrial translation and cell cycle checkpoint control. We show that fission yeast has more essential genes than budding yeast and that essential genes are more likely than nonessential genes to be present in a single copy, to be broadly conserved and to contain introns. Growth fitness analyses determined sets of haploinsufficient and haploproficient genes for fission yeast, and comparisons with budding yeast identified specific ribosomal proteins and RNA polymerase subunits, which may act more generally to regulate eukaryotic cell growth.

PMID:
20473289
PMCID:
PMC3962850
DOI:
10.1038/nbt.1628
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center