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Exp Toxicol Pathol. 2011 Sep;63(6):549-52. doi: 10.1016/j.etp.2010.04.006. Epub 2010 May 14.

Increased oxidative stress in dystrophin deficient (mdx) mice masticatory muscles.

Author information

1
Department of Orthodontics, Faculty of Medicine, University of Greifswald, Greifswald, Germany. alexspas@uni-greifswald.de

Abstract

BACKGROUND:

It has been suggested that increased oxidative stress and the glutathione antioxidant system play an important role in the pathogenesis of Duchenne muscular dystrophy. However, there is still a lack of data about the oxidative status in dystrophic masticatory muscles.

METHODS:

In the masticatory muscles of the mouse model of Duchenne muscular dystrophy (mdx and controls; 100 days old, n=8-10 each group) we examined the GSH and GSSG content (glutathione reduced/oxidized form) and the level of lipid peroxidation (LPO) as measured by the thiobarbituric acid-reaction.

RESULTS:

In the mdx mice masticatory muscles we found increased oxidative stress as compared to the controls. The GSH values in mdx muscles were decreased (mean±SEM; masseter 339.8±37.6 μg/g vs. 523.1±36.1 μg/g, temporal 304.1±49.6 μg/g vs.512.6±60.6 μg/g, tongue muscle 243.3±28. 8 μg/g vs. 474.9±40.1 μg/g; Fig. 1) as compared to normal mice. The GSH/GSSG ratio in mdx mice was consequently decreased. No significant differences in GSSG content and LPO levels were found between mdx and control mice.

CONCLUSIONS:

The results imply that oxidative stress is present in all three studied mdx mouse masticatory muscles.

PMID:
20471229
DOI:
10.1016/j.etp.2010.04.006
[Indexed for MEDLINE]

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