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Biochem Biophys Res Commun. 2010 Jun 18;397(1):18-21. doi: 10.1016/j.bbrc.2010.05.036. Epub 2010 May 12.

Stepwise oxygenations of toluene and 4-nitrotoluene by a fungal peroxygenase.

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Unit of Environmental Biotechnology, International Graduate School of Zittau, Markt 23, Zittau, Germany.


Fungal peroxygenases have recently been shown to catalyze remarkable oxidation reactions. The present study addresses the mechanism of benzylic oxygenations catalyzed by the extracellular peroxygenase of the agaric basidiomycete Agrocybe aegerita. The peroxygenase oxidized toluene and 4-nitrotoluene via the corresponding alcohols and aldehydes to give benzoic acids. The reactions proceeded stepwise with total conversions of 93% for toluene and 12% for 4-nitrotoluene. Using H(2)(18)O(2) as the co-substrate, we show here that H(2)O(2) is the source of the oxygen introduced at each reaction step. A. aegerita peroxygenase resembles cytochromes P450 and heme chloroperoxidase in catalyzing benzylic hydroxylations.

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