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EMBO Rep. 2010 Jun;11(6):445-51. doi: 10.1038/embor.2010.64. Epub 2010 May 14.

Direct interaction between hnRNP-M and CDC5L/PLRG1 proteins affects alternative splice site choice.

Author information

1
Wellcome Trust Centre for Gene Regulation & Expression, College of Life Sciences, University of Dundee, MSI/WTB/JBC Complex, Dow Street, Dundee DD1 5EH, UK.

Abstract

Heterogeneous nuclear ribonucleoprotein-M (hnRNP-M) is an abundant nuclear protein that binds to pre-mRNA and is a component of the spliceosome complex. A direct interaction was detected in vivo between hnRNP-M and the human spliceosome proteins cell division cycle 5-like (CDC5L) and pleiotropic regulator 1 (PLRG1) that was inhibited during the heat-shock stress response. A central region in hnRNP-M is required for interaction with CDC5L/PLRG1. hnRNP-M affects both 5' and 3' alternative splice site choices, and an hnRNP-M mutant lacking the CDC5L/PLRG1 interaction domain is unable to modulate alternative splicing of an adeno-E1A mini-gene substrate.

PMID:
20467437
PMCID:
PMC2892320
DOI:
10.1038/embor.2010.64
[Indexed for MEDLINE]
Free PMC Article

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