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Biotechnol Lett. 2010 Sep;32(9):1339-46. doi: 10.1007/s10529-010-0293-x. Epub 2010 May 13.

Chondrogenic differentiation of human mesenchymal stem cells: a comparison between micromass and pellet culture systems.

Author information

1
Department of Orthopedics, Second Affiliated Hospital, Sun Yat-Sen University, #107 Yanjiang Road West, Guangzhou 510120, China.

Abstract

High-density cell culture is pivotal for the chondrogenic differentiation of human mesenchymal stem cells (hMSCs). Two high-density cell culture systems, micromass and pellet culture, have been used to induce chondrogenic differentiation of hMSCs. In micromass culture, the induced-cartilage tissues were larger, more homogenous and enriched in cartilage-specific collagen II but the fibrocartilage-like feature, collagen I, and hypertrophic chondrocyte feature, collagen X, were markedly decreased compared to those in pellet culture. Furthermore, real time RT-PCR analysis demonstrated that collagen II and aggrecan mRNA were up-regulated while collagen X and collagen I mRNA were down-regulated in micromass culture. Thus, the micromass culture system is a promising tool for in vitro chondrogenic studies.

PMID:
20464452
DOI:
10.1007/s10529-010-0293-x
[Indexed for MEDLINE]

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