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BMC Infect Dis. 2010 May 11;10:113. doi: 10.1186/1471-2334-10-113.

Rapid semi-automated quantitative multiplex tandem PCR (MT-PCR) assays for the differential diagnosis of influenza-like illness.

Author information

1
Centre for Infectious Diseases and Microbiology, University of Sydney, Westmead Hospital, Westmead NSW 2145, Australia.

Abstract

BACKGROUND:

Influenza A, including avian influenza, is a major public health threat in developed and developing countries. Rapid and accurate detection is a key component of strategies to contain spread of infection, and the efficient diagnosis of influenza-like-illness is essential to protect health infrastructure in the event of a major influenza outbreak.

METHODS:

We developed a multiplexed PCR (MT-PCR) assay for the simultaneous diagnosis of respiratory viruses causing influenza-like illness, including the specific recognition of influenza A haemagglutinin subtypes H1, H3, and H5. We tested several hundred clinical specimens in two diagnostic reference laboratories and compared the results with standard techniques.

RESULTS:

The sensitivity and specificity of these assays was higher than individual assays based on direct antigen detection and standard PCR against a range of control templates and in several hundred clinical specimens. The MT-PCR assays provided differential diagnoses as well as potentially useful quantitation of virus in clinical samples.

CONCLUSIONS:

MT-PCR is a potentially powerful tool for the differential diagnosis of influenza-like illness in the clinical diagnostic laboratory.

PMID:
20459845
PMCID:
PMC2881921
DOI:
10.1186/1471-2334-10-113
[Indexed for MEDLINE]
Free PMC Article

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