Format

Send to

Choose Destination
Virology. 2010 Aug 1;403(2):145-54. doi: 10.1016/j.virol.2010.04.014. Epub 2010 May 10.

Characterization of the gene expression profile of human bocavirus.

Author information

1
Department of Microbiology, University of Kansas Medical Center, Kansas City, KS, USA.

Abstract

We have generated a quantitative transcription profile of human bocavirus type 1 (HBoV1) by transfecting a nearly full-length clone in human lung epithelial A549 cells as well as in a replication competent system in 293 cells. The overall transcription profile of HBoV1 is similar to that of two other members of genus Bocavirus, minute virus of canines and bovine parvovirus 1. In particular, a spliced NS1-transcript that was not recognized previously expressed the large non-structural protein NS1 at approximately 100kDa; and the NP1-encoding transcripts were expressed abundantly. In addition, the protein expression profile of human bocavirus type 2 (HBoV2) was examined in parallel by transfection of a nearly full-length clone in A549 cells, which is similar to that of HBoV1. Moreover, our results showed that, unlike human parvovirus B19 infection, expression of the HBoV1 proteins only does not induce cell cycle arrest and apoptosis of A549 cells.

PMID:
20457462
PMCID:
PMC2879452
DOI:
10.1016/j.virol.2010.04.014
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center