Format

Send to

Choose Destination
See comment in PubMed Commons below
J Voice. 2011 Mar;25(2):249-53. doi: 10.1016/j.jvoice.2009.10.003. Epub 2010 Apr 24.

In Vivo engineering of the vocal fold ECM with injectable HA hydrogels-late effects on tissue repair and biomechanics in a rabbit model.

Author information

1
Department of Surgery, Division of Otolaryngology-Head and Neck Surgery, University of Wisconsin Madison, Madison, Wisconsin, USA. sarah-klemuk@uiowa.edu

Abstract

OBJECTIVES:

To determine if the utilization of injectable chemically modified hyaluronan (HA) derivative at the time of intentional vocal fold resection may facilitate wound repair and preserve the unique viscoelastic properties of the extracellular matrix (ECM) and lamina propria 6 months after treatment.

STUDY DESIGN:

Prospective, controlled animal study.

METHODS:

Twelve rabbit vocal folds were biopsied bilaterally, and the left side of vocal fold was treated with Extracel, an injectable, chemically modified HA derivative, and the right side of vocal fold was injected with saline as control at the time of resection. Animals were sacrificed 6 months after biopsy and injection. Outcomes measured include transcription levels for procollagen, fibronectin, fibromodulin, transforming growth factor beta one (TGF-β1), HA synthase, and hyaluronidase, and tissue biomechanics-viscosity and elasticity.

RESULTS:

Extracel-treated vocal folds were found to have significantly less fibrosis than saline-treated controls. Extracel-treated vocal folds had significantly improved biomechanical properties of elasticity and viscosity. Significantly decreased levels of fibronectin, fibromodulin, TGF-β1, procollagen I, and HA synthase were measured.

CONCLUSIONS:

Prophylactic in vivo manipulation of the ECM with an injectable HA hydrogel appears to induce vocal fold tissue regeneration to yield improved tissue composition and biomechanical properties at 6 months.

PMID:
20456912
PMCID:
PMC2921026
DOI:
10.1016/j.jvoice.2009.10.003
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for PubMed Central
    Loading ...
    Support Center