Format

Send to

Choose Destination
See comment in PubMed Commons below
Proteomics. 2010 Jul;10(14):2712-8. doi: 10.1002/pmic.200900473.

Computational analysis of unassigned high-quality MS/MS spectra in proteomic data sets.

Author information

1
Department of Pathology, University of Michigan, Ann Arbor, MI, USA.

Abstract

In a typical shotgun proteomics experiment, a significant number of high-quality MS/MS spectra remain "unassigned." The main focus of this work is to improve our understanding of various sources of unassigned high-quality spectra. To achieve this, we designed an iterative computational approach for more efficient interrogation of MS/MS data. The method involves multiple stages of database searching with different search parameters, spectral library searching, blind searching for modified peptides, and genomic database searching. The method is applied to a large publicly available shotgun proteomic data set.

PMID:
20455209
PMCID:
PMC3517130
DOI:
10.1002/pmic.200900473
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Wiley Icon for PubMed Central
    Loading ...
    Support Center