**a** Normalized I_{ClVol} recorded at +120 mV from 4 different cells bathed and dialyzed with solutions containing 25, 70, 140 and 200 mM Cl^{-}. Hypotonic solutions used to activate I_{ClVol} were prepared by removing D-mannitol from the corresponding isotonic solutions. **b** Normalized I-V relationships at the indicated [Cl]. I_{ClVol} magnitudes were measured at the end of each voltage step and normalized to the I_{ClVol} magnitude measured at -80 mV. **c** FIC vs V_{m} curves as a function of [Cl^{-}]_{i} = [Cl^{-}]_{e} = 200 (*black circles*, n=6), 140 (*down triangles*, n=12), 70 (*up triangles*, n=10) and 25 mM (*black boxes*, n=4). Solutions used in panels a, b and c were A_{i}, A_{e1}, A_{e2}, A_{e3}, F_{i}, F_{e1}, F_{e2}, F_{e3}, G_{i}, G_{e1}, G_{e2}, G_{e3}, H_{i}, H_{e1}, H_{e2} and H_{e3}. **d** FIC vs V_{m} curves as a function of [Cl^{-}]_{i} (140mM, *up triangles*, n=15; 70 mM, *black circles*, n=9, or 25 mM, *black boxes*, n=6) when [Cl^{-}]_{e} was 140 mM. Continuous lines are fits with . Solutions used: A_{i}, A_{e1},Ji and K_{i}. **e** Curves of FIC vs V_{m} at [Cl^{-}]_{e} of 140 (*black boxes*, n=12) or 25 mM (*up triangles*, n=9). In all cases [Cl^{-}]_{i} was 140 mM while pH_{i} = pH_{e} = 7.3 (solutions A_{i}, A_{e1} and I_{e2}).

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