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Appl Environ Microbiol. 2010 Jul;76(13):4560-5. doi: 10.1128/AEM.00515-10. Epub 2010 May 7.

Pilot-scale production of fatty acid ethyl esters by an engineered Escherichia coli strain harboring the p(Microdiesel) plasmid.

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  • 1Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, Corrensstrasse 3, D-48149 Munster, Germany.

Abstract

Fatty acid ethyl esters (FAEEs) were produced in this study by the use of an engineered Escherichia coli p(Microdiesel) strain. Four fed-batch pilot scale cultivations were carried out by first using glycerol as sole carbon source for biomass production before glucose and oleic acid were added as carbon sources. Cultivations yielded a cell density of up to 61 +/- 3.1 g of cell dry mass (CDM) per liter and a maximal FAEE content of 25.4% +/- 1.1% (wt/wt) of CDM.

PMID:
20453138
PMCID:
PMC2897437
DOI:
10.1128/AEM.00515-10
[PubMed - indexed for MEDLINE]
Free PMC Article
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