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Carbohydr Res. 2010 Jun 16;345(9):1180-9. doi: 10.1016/j.carres.2010.03.042. Epub 2010 Apr 4.

Branched arabino-oligosaccharides isolated from sugar beet arabinan.

Author information

1
Wageningen University, Laboratory of Food Chemistry, Wageningen, The Netherlands.

Abstract

Sugar beet arabinan consists of an alpha-(1,5)-linked backbone of L-arabinosyl residues, which can be either single or double substituted with alpha-(1,2)- and/or alpha-(1,3)-linked L-arabinosyl residues. Neutral branched arabino-oligosaccharides were isolated from sugar beet arabinan by enzymatic degradation with mixtures of pure and well-defined arabinohydrolases from Chrysosporium lucknowense followed by fractionation based on size and analysis by MALDI-TOF MS and HPAEC. Using NMR analysis, two main series of branched arabino-oligosaccharides have been identified, both having an alpha-(1,5)-linked backbone of L-arabinosyl residues. One series carries single substituted alpha-(1,3)-linked L-arabinosyl residues at the backbone, whereas the other series consists of a double substituted alpha-(1,2,3,5)-linked arabinan structure within the molecule. The structures of eight such branched arabino-oligosaccharides were established.

PMID:
20452576
DOI:
10.1016/j.carres.2010.03.042
[Indexed for MEDLINE]

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