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Cell Stem Cell. 2010 May 7;6(5):468-78. doi: 10.1016/j.stem.2010.03.015.

Tracing the derivation of embryonic stem cells from the inner cell mass by single-cell RNA-Seq analysis.

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1
Wellcome Trust/Cancer Research, UK.

Abstract

During the transition from the inner cell mass (ICM) cells of blastocysts to pluripotent embryonic stem cells (ESCs) in vitro, a normal developmental program is replaced in cells that acquire a capacity for infinite self-renewal and pluripotency. We explored the underlying mechanism of this switch by using RNA-Seq transcriptome analysis at the resolution of single cells. We detected significant molecular transitions and major changes in transcript variants, which include genes for general metabolism. Furthermore, the expression of repressive epigenetic regulators increased with a concomitant decrease in gene activators that might be necessary to sustain the inherent plasticity of ESCs. Furthermore, we detected changes in microRNAs (miRNAs), with one set that targets early differentiation genes while another set targets pluripotency genes to maintain the unique ESC epigenotype. Such genetic and epigenetic events may contribute to a switch from a normal developmental program in adult cells during the formation of diseased tissues, including cancers.

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PMID:
20452321
PMCID:
PMC2954317
DOI:
10.1016/j.stem.2010.03.015
[Indexed for MEDLINE]
Free PMC Article

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