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J Microbiol Methods. 2010 Aug;82(2):184-6. doi: 10.1016/j.mimet.2010.04.011. Epub 2010 May 6.

A simple and fast method to analyze the orientation of c-type cytochromes in the outer membrane of Gram-negative bacteria.

Author information

1
Department of Microbiology, University of Freiburg, D-79104 Freiburg, Germany.

Abstract

Outer membrane cytochromes catalyze the final reduction step of respiratory chains to electron acceptors that cannot diffuse through the outer membrane of Gram-negative bacteria. We developed an in vivo method to detect the orientation of outer membrane cytochromes via analysis of electron transfer reactions between these enzymes and riboflavin.

PMID:
20451567
DOI:
10.1016/j.mimet.2010.04.011
[Indexed for MEDLINE]

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