Abstract
The complete amino acid sequence of bovine spleen cathepsin S has been determined. The single-chain protein contains 217 residues and has a Mr of 23,682. The primary structure was determined by sequencing of native protein and the peptides obtained by proteolytic cleavage with beta-trypsin, papaya proteinase IV and by chemical cleavage with cyanogen bromide. Comparison of the amino terminal sequences of the heavy and the light chain of bovine cathepsin L with that of bovine cathepsin S clearly indicates that the enzymes are structurally different.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Cathepsin L
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Cathepsins / chemistry*
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Cathepsins / genetics
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Cathepsins / isolation & purification
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Cattle
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Cysteine Endopeptidases
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Endopeptidases*
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Humans
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Molecular Sequence Data
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Peptide Fragments / isolation & purification
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Sequence Homology, Nucleic Acid
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Spleen / enzymology
Substances
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Peptide Fragments
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Cathepsins
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Endopeptidases
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Cysteine Endopeptidases
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CTSL protein, human
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Cathepsin L
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cathepsin S
Associated data
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GENBANK/M90696
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GENBANK/M95211
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GENBANK/X58797
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GENBANK/X58798
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GENBANK/X58799
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GENBANK/X58800
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GENBANK/X58801
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GENBANK/X58802
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GENBANK/X58803
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GENBANK/X62841