The complete amino acid sequence of bovine cathepsin S and a partial sequence of bovine cathepsin L

FEBS Lett. 1991 Jun 3;283(2):329-31. doi: 10.1016/0014-5793(91)80620-i.

Abstract

The complete amino acid sequence of bovine spleen cathepsin S has been determined. The single-chain protein contains 217 residues and has a Mr of 23,682. The primary structure was determined by sequencing of native protein and the peptides obtained by proteolytic cleavage with beta-trypsin, papaya proteinase IV and by chemical cleavage with cyanogen bromide. Comparison of the amino terminal sequences of the heavy and the light chain of bovine cathepsin L with that of bovine cathepsin S clearly indicates that the enzymes are structurally different.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cathepsin L
  • Cathepsins / chemistry*
  • Cathepsins / genetics
  • Cathepsins / isolation & purification
  • Cattle
  • Cysteine Endopeptidases
  • Endopeptidases*
  • Humans
  • Molecular Sequence Data
  • Peptide Fragments / isolation & purification
  • Sequence Homology, Nucleic Acid
  • Spleen / enzymology

Substances

  • Peptide Fragments
  • Cathepsins
  • Endopeptidases
  • Cysteine Endopeptidases
  • CTSL protein, human
  • Cathepsin L
  • cathepsin S

Associated data

  • GENBANK/M90696
  • GENBANK/M95211
  • GENBANK/X58797
  • GENBANK/X58798
  • GENBANK/X58799
  • GENBANK/X58800
  • GENBANK/X58801
  • GENBANK/X58802
  • GENBANK/X58803
  • GENBANK/X62841