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Plant Cell Environ. 2010 Oct;33(10):1641-1655. doi: 10.1111/j.1365-3040.2010.02170.x.

Differentiation of metallicolous and non-metallicolous Salix caprea populations based on phenotypic characteristics and nuclear microsatellite (SSR) markers.

Author information

1
Institute of Soil Science, Department of Forest and Soil Sciences, BOKU-University of Natural Resources and Applied Life Sciences.
2
Department of Applied Genetics and Cell Biology, BOKU-University of Natural Resources and Applied Life Sciences.
3
Department of Landscape, Spatial and Infrastructure Sciences, University of Natural Resources and Applied Life Sciences, A-1190 Vienna, Austria.
#
Contributed equally

Abstract

The Salicaceae family comprises a large number of high-biomass species with remarkable genetic variability and adaptation to ecological niches. Salix caprea survives in heavy metal contaminated areas, translocates and accumulates Zn/Cd in leaves. To reveal potential selective effects of long-term heavy metal contaminations on the genetic structure and Zn/Cd accumulation capacity, 170 S. caprea isolates of four metal-contaminated and three non-contaminated middle European sites were analysed with microsatellite markers using Wright's F statistics. The differentiation of populations North of the Alps are more pronounced compared to the Southern ones. By grouping the isolates based on their contamination status, a weak but significant differentiation was calculated between Northern metallicolous and non-metallicolous populations. To quantify if the contamination and genetic status of the populations correlate with Zn/Cd tolerance and the accumulation capacity, the S. caprea isolates were exposed to elevated Cd/Zn concentrations in perlite-based cultures. Consistent with the genetic data nested anova analyses for the physiological traits find a significant difference in the Cd accumulation capacity between the Northern and Southern populations. Our data suggest that natural populations are a profitable source to uncover genetic mechanisms of heavy metal accumulation and biomass production, traits that are essential for improving phytoextraction strategies.

PMID:
20444221
PMCID:
PMC4361689
DOI:
10.1111/j.1365-3040.2010.02170.x
[Indexed for MEDLINE]
Free PMC Article

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