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Res Microbiol. 2010 Jun;161(5):363-71. doi: 10.1016/j.resmic.2010.04.002. Epub 2010 May 14.

RcsB plays a central role in H-NS-dependent regulation of motility and acid stress resistance in Escherichia coli.

Author information

1
Institut Pasteur, Unité de Génétique des Génomes Bactériens, CNRS URA2171, 28 Rue du Docteur Roux, 75724 Paris Cedex 15, France. ekrin@pasteur.fr

Abstract

In Escherichia coli, hns mutants lack flagellar motility and display an increase in acid stress resistance. Spontaneous phenotypic revertants showed reversion of both H-NS-controlled phenotypes. In the present study, suppressor mutations were identified in the rcsB gene. In addition to RcsA, our experiments establish that H-NS indirectly controlled the RcsB regulator via repression of RcsD. We also show that RcsB(D56E), mimicking phosphorylated RcsB, interacts with GadE to form a RcsB-P/GadE complex, a general direct regulator of glutamate-, arginine- and lysine-dependent acid resistance pathways. In addition, we showed that H-NS positively affects motility via the flhDC master operon repression by RcsB. This substantiates the central role of RcsB in H-NS-mediated control of motility and acid stress resistance.

PMID:
20435136
DOI:
10.1016/j.resmic.2010.04.002
[Indexed for MEDLINE]

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