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Toxicology. 2010 Jun 29;273(1-3):29-34. doi: 10.1016/j.tox.2010.04.013. Epub 2010 May 7.

The role of protein kinase C in the opening of blood-brain barrier induced by electromagnetic pulse.

Author information

1
Department of Radiation Medicine, Faculty of Preventive Medicine, Fourth Military Medical University, Xi'an, China.

Abstract

The aim of this study was to determine the role of protein kinase C signaling in electromagnetic pulse (EMP)-induced blood-brain barrier (BBB) permeability change in rats. The protein level of total PKC and two PKC isoforms (PKC-alpha, and PKC-beta II) were determined in brain cerebral cortex microvessels by Western blot after exposing rats to EMP at 200kV/m for 200 pulses with 1Hz repetition rate. It was found that the protein level of PKC and PKC-betaII (but not PKC-alpha) in cerebral cortex microvessels increased significantly at 0.5h and 1h after EMP exposure compared with sham-exposed animals and then recovered at 3h. A specific PKC antagonist (H7) almost blocked EMP-induced BBB permeability change. EMP-induced BBB tight junction protein ZO-1 translocation was also inhibited. Our data indicated that PKC signaling was involved in EMP-induced BBB permeability change and ZO-1 translocation in rat.

PMID:
20435084
DOI:
10.1016/j.tox.2010.04.013
[Indexed for MEDLINE]

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