Format

Send to

Choose Destination
Biochem Cell Biol. 1991 Jan;69(1):72-8.

Structure of the glycan chain from the surface layer glycoprotein of Bacillus alvei CCM 2051.

Author information

1
Institute for Biological Sciences, National Research Council, Ottawa, Ont., Canada.

Abstract

The cell surface of the mesophilic eubacterium Bacillus alvei CCM 2051 is covered by an oblique arranged surface layer glycoprotein. The subunits revealed by sodium dodecyl sulfate - polyacrylamide gel electrophoresis were distinct bands of molecular masses 140,000, 128,000, and 127,000. Proteolytic degradation of the purified S-layer glycoprotein yielded a single glycopeptide fraction with an apparent molecular mass of ca. 25,000. Methylation analysis in conjunction with two-dimensional nuclear magnetic resonance experiments at 500 MHz established the branched trisaccharide (formula; see text) as the repeating unit for this glycan chain.

PMID:
2043344
DOI:
10.1139/o91-010
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Atypon
Loading ...
Support Center