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Am J Obstet Gynecol. 2010 Jun;202(6):592.e1-7. doi: 10.1016/j.ajog.2010.03.002. Epub 2010 Apr 28.

Overexpression of miR-152 leads to reduced expression of human leukocyte antigen-G and increased natural killer cell mediated cytolysis in JEG-3 cells.

Author information

1
Department of Obstetrics and Gynecology, Tangdu Hospital, The Fourth Military Medical University, Xi'an, China. xiaomingzhu1981@hotmail.com

Abstract

OBJECTIVE:

The purpose of this study was to gain a further understanding of the relationship between miR-152 and human leukocyte antigen (HLA)-G in human trophoblast cell line (JEG-3).

STUDY DESIGN:

The JEG-3 cells were transfected with pre-miR-152. The effect of the overexpressed miR-152 on HLA-G expression, trophoblast invasion, and natural killer (NK) cell-mediated cytolysis were assessed by reverse-transcription polymerase chain reaction (RT-PCR) and Western blot analysis, transwell invasion assay, and NK cell cytotoxicity assay, respectively.

RESULTS:

The miR-152 repressed HLA-G expression but exerted no effect on JEG-3 cell invasion, and overexpression of miR-152 led to increased NK cell-mediated cytolysis in JEG-3 cells.

CONCLUSION:

The data indicate that miR-152 may function as an immune system enhancer through up-regulating NK cell-mediated cytolysis of host cells.

PMID:
20430358
DOI:
10.1016/j.ajog.2010.03.002
[Indexed for MEDLINE]

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