Format

Send to

Choose Destination
See comment in PubMed Commons below
Biochem Biophys Res Commun. 2010 May 28;396(2):382-7. doi: 10.1016/j.bbrc.2010.04.101. Epub 2010 Apr 27.

Genomic analysis of the telomeric length effect on organismic lifespan in Caenorhabditis elegans.

Author information

  • 1Department of Biological Sciences, Research Center of Functional Cellulomics, Institute of Molecular Biology and Genetics, Seoul National University, Seoul, Republic of Korea.

Abstract

Telomeres are multifunctional elements that protect chromosomal ends. Telomere length affects lifespan and stress resistance. In our previous report, we found that the increase of telomere length extended organismic lifespan in Caenorhabditis elegans. To investigate the effect of the telomere length on organismic lifespan at the global gene expression level, we performed microarray experiments using mRNA from long telomere-containing worms. We identified genes that showed significant difference in transcript levels from long telomere-containing worms.We examined the expression patterns of these genes and measured lifespan of the worms over-expressing each of the up-regulated genes. Most up-regulated genes, when over-expressed, extended lifespan of C. elegans. In particular, over-expression of rnh-1.3 in the intestine extended lifespan in the daf-16-dependent manner and increased stress resistance. Interestingly, genes up-regulated in long telomeric worms partially overlapped with the genes whose transcription was increased by the rde-3 mutations, and the over-expression of each of these genes also extended the longevity of worms, raising the possibility that the telomere effect may involve molecular players shared with the RNAi machinery.

PMID:
20430011
DOI:
10.1016/j.bbrc.2010.04.101
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center