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Mol Biol Cell. 2010 Jun 15;21(12):1968-81. doi: 10.1091/mbc.E09-04-0356. Epub 2010 Apr 28.

Spindly/CCDC99 is required for efficient chromosome congression and mitotic checkpoint regulation.

Author information

1
Division of Molecular Pathophysiology, Biocenter, Innsbruck Medical University, 6020 Innsbruck, Austria.

Abstract

Spindly recruits a fraction of cytoplasmic dynein to kinetochores for poleward movement of chromosomes and control of mitotic checkpoint signaling. Here we show that human Spindly is a cell cycle-regulated mitotic phosphoprotein that interacts with the Rod/ZW10/Zwilch (RZZ) complex. The kinetochore levels of Spindly are regulated by microtubule attachment and biorientation induced tension. Deletion mutants lacking the N-terminal half of the protein (NDelta253), or the conserved Spindly box (DeltaSB), strongly localized to kinetochores and failed to respond to attachment or tension. In addition, these mutants prevented the removal of the RZZ complex and that of MAD2 from bioriented chromosomes and caused cells to arrest at metaphase, showing that RZZ-Spindly has to be removed from kinetochores to terminate mitotic checkpoint signaling. Depletion of Spindly by RNAi, however, caused cells to arrest in prometaphase because of a delay in microtubule attachment. Surprisingly, this defect was alleviated by codepletion of ZW10. Thus, Spindly is not only required for kinetochore localization of dynein but is a functional component of a mechanism that couples dynein-dependent poleward movement of chromosomes to their efficient attachment to microtubules.

PMID:
20427577
PMCID:
PMC2883941
DOI:
10.1091/mbc.e09-04-0356
[Indexed for MEDLINE]
Free PMC Article

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