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Appl Biochem Biotechnol. 2010 Nov;162(6):1785-803. doi: 10.1007/s12010-010-8959-3. Epub 2010 Apr 27.

Effect of extruder parameters and moisture content of switchgrass, prairie cord grass on sugar recovery from enzymatic hydrolysis.

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1
Agricultural and Biosystems Engineering Department, South Dakota State University, 1400 North Campus Drive, Brookings, SD 57007, USA. Chinnadurai.karunani@sdstate.edu

Abstract

Research on biomass pretreatment to enhance enzymatic digestibility has been done for more than decades, but a viable continuous pretreatment method needs to be developed. Extrusion has the potential to be a viable continuous pretreatment method. This study investigated the effect of compression ratio (2:1 and 3:1), screw speed (50, 100, and 150 rpm), and barrel temperature (50, 100, and 150 degrees C) on the sugar recovery from switchgrass (SG) and prairie cord grass (PCG) over a range of moisture contents (15, 25, 35, and 45% wb).The pretreated samples were subjected to enzymatic hydrolysis for sugar recovery measurement. Statistical analyses revealed that a 3:1 screw compression ratio (compared to 2:1) increased glucose recovery by 12% and 8% and combined sugar recovery by 37% and 40% for SG and PCG, respectively. For SG, the highest sugar recovery (45.2%) was obtained at the lowest screw speed (50 rpm) and the highest temperature (150 degrees C) with moisture content of 15%. The highest glucose, xylose, and combined sugar recovery of 61.4%, 84.3%, and 65.8% were recorded for PCG extruded at a screw speed of 50 rpm and a temperature of 50 degrees C with a moisture content of 25%. Glycerol and acetic acid were byproducts found in low concentration (0.02-0.18 g/L) for both biomass.

PMID:
20419510
DOI:
10.1007/s12010-010-8959-3
[Indexed for MEDLINE]
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