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Methods Mol Biol. 2010;637:311-31. doi: 10.1007/978-1-60761-700-6_17.

Measurement of Intracellular pH.

Author information

1
CIHR Membrane Protein Research Group, Departments of Physiology and Biochemistry, University of Alberta Edmonton, Edmonton, AB, Canada.

Abstract

The activity of most cellular processes is sensitive to pH. Cells therefore tightly control cytosol pH within narrow bounds. Measurement of cytosolic pH is of interest in studying many processes, including pH regulatory transport proteins. Key approaches that have been used to determine intracellular pH include pH-sensitive microelectrodes, nuclear magnetic resonance, and pH-sensitive fluorescent proteins. Here we review these approaches while providing details on the use of pH-sensitive fluorescent dyes to measure cytosolic pH.

PMID:
20419443
DOI:
10.1007/978-1-60761-700-6_17
[Indexed for MEDLINE]

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