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PLoS One. 2010 Apr 14;5(4):e10176. doi: 10.1371/journal.pone.0010176.

Specific recognition of influenza A/H1N1/2009 antibodies in human serum: a simple virus-free ELISA method.

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  • 1Centro de Biotecnología-FEMSA, Tecnológico de Monterrey, Monterrey, New León, México. mario.alvarez@itesm.mx

Abstract

BACKGROUND:

Although it has been estimated that pandemic Influenza A H1N1/2009 has infected millions of people from April to October 2009, a more precise figure requires a worldwide large-scale diagnosis of the presence of Influenza A/H1N1/2009 antibodies within the population. Assays typically used to estimate antibody titers (hemagglutination inhibition and microneutralization) would require the use of the virus, which would seriously limit broad implementation.

METHODOLOGY/PRINCIPAL FINDINGS:

An ELISA method to evaluate the presence and relative concentration of specific Influenza A/H1N1/2009 antibodies in human serum samples is presented. The method is based on the use of a histidine-tagged recombinant fragment of the globular region of the hemagglutinin (HA) of the Influenza A H1N1/2009 virus expressed in E. coli.

CONCLUSIONS/SIGNIFICANCE:

The ELISA method consistently discerns between Inf A H1N1 infected and non-infected subjects, particularly after the third week of infection/exposure. Since it does not require the use of viral particles, it can be easily and quickly implemented in any basic laboratory. In addition, in a scenario of insufficient vaccine availability, the use of this ELISA could be useful to determine if a person has some level of specific antibodies against the virus and presumably at least partial protection.

PMID:
20418957
PMCID:
PMC2854694
DOI:
10.1371/journal.pone.0010176
[PubMed - indexed for MEDLINE]
Free PMC Article
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