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PLoS One. 2010 Apr 14;5(4):e10177. doi: 10.1371/journal.pone.0010177.

High-content, image-based screening for drug targets in yeast.

Author information

1
Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba, Japan.

Abstract

BACKGROUND:

Drug discovery and development are predicated on elucidation of the potential mechanisms of action and cellular targets of candidate chemical compounds. Recent advances in high-content imaging techniques allow simultaneous analysis of a range of cellular events. In this study, we propose a novel strategy to identify drug targets by combining genetic screening and high-content imaging in yeast.

METHODOLOGY:

In this approach, we infer the cellular functions affected by candidate drugs by comparing morphologic changes induced by the compounds with the phenotypes of yeast mutants.

CONCLUSIONS:

Using this method and four well-characterized reagents, we successfully identified previously known target genes of the compounds as well as other genes involved with functionally related cellular pathways. This is the first demonstration of a genetic high-content assay that can be used to identify drug targets based on morphologic phenotypes of a reference mutant panel.

PMID:
20418956
PMCID:
PMC2854693
DOI:
10.1371/journal.pone.0010177
[Indexed for MEDLINE]
Free PMC Article

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