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Nat Methods. 2010 Jun;7(6):451-3. doi: 10.1038/nmeth.1454. Epub 2010 Apr 25.

Targeted gene deletions in C. elegans using transposon excision.

Author information

1
Howard Hughes Medical Institute, Department of Biology, University of Utah, Salt Lake City, Utah, USA.

Abstract

We developed a method, MosDEL, to generate targeted knockouts of genes in Caenorhabditis elegans by injection. We generated a double-strand break by mobilizing a Mos1 transposon adjacent to the region to be deleted; the double-stranded break is repaired using injected DNA as a template. Repair can delete up to 25 kb of DNA and simultaneously insert a positive selection marker.

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PMID:
20418868
PMCID:
PMC2878396
DOI:
10.1038/nmeth.1454
[Indexed for MEDLINE]
Free PMC Article

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