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Appl Environ Microbiol. 2010 Jun;76(12):4092-5. doi: 10.1128/AEM.03034-09. Epub 2010 Apr 23.

Species-specific type II restriction-modification system of Xylella fastidiosa temecula1.

Author information

1
Department of Microbiology, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.

Abstract

The transformation efficiency of Xylella fastidiosa can be increased by interfering with restriction by the strain-specific type II system encoded by the PD1607 and PD1608 genes. Here, we report results for two strategies: in vitro methylation using M.SssI and isolation of DNA from an Escherichia coli strain expressing the methylase PD1607.

PMID:
20418439
PMCID:
PMC2893502
DOI:
10.1128/AEM.03034-09
[Indexed for MEDLINE]
Free PMC Article

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