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J Mol Biol. 2010 Jun 11;399(3):501-11. doi: 10.1016/j.jmb.2010.04.029. Epub 2010 Apr 22.

Monomeric rhodopsin is the minimal functional unit required for arrestin binding.

Author information

1
Department of Biochemistry and Molecular Biology, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239-3098, USA.

Abstract

We have tested whether arrestin binding requires the G-protein-coupled receptor be a dimer or a multimer. To do this, we encapsulated single-rhodopsin molecules into nanoscale phospholipid particles (so-called nanodiscs) and measured their ability to bind arrestin. Our data clearly show that both visual arrestin and beta-arrestin 1 can bind to monomeric rhodopsin and stabilize the active metarhodopsin II form. Interestingly, we find that the monomeric rhodopsin in nanodiscs has a higher affinity for wild-type arrestin binding than does oligomeric rhodopsin in liposomes or nanodiscs, as assessed by stabilization of metarhodopsin II. Together, these results establish that rhodopsin self-association is not required to enable arrestin binding.

PMID:
20417217
PMCID:
PMC3848883
DOI:
10.1016/j.jmb.2010.04.029
[Indexed for MEDLINE]
Free PMC Article

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