Format

Send to

Choose Destination
Biochim Biophys Acta. 2010 Aug;1797(8):1470-6. doi: 10.1016/j.bbabio.2010.04.008. Epub 2010 Apr 21.

The role of UCP 1 in production of reactive oxygen species by mitochondria isolated from brown adipose tissue.

Author information

1
School of Biochemistry and Immunology, Trinity College Dublin, Dublin 2, Ireland.

Abstract

We provide evidence that ablation or inhibition of, uncoupling protein 1 increases the rate of reactive oxygen containing species production by mitochondria from brown adipose tissue, no matter what electron transport chain substrate is used (succinate, glycerol-3-phosphate or pyruvate/malate). Consistent with these data are our observations that (a) the mitochondrial membrane potential is maximal when uncoupling protein 1 is ablated or inhibited and (b) oxygen consumption rates in mitochondria from uncoupling protein 1 knock-out mice, are significantly lower than those from wild-type mice, but equivalent to those from wild-type mice in the presence of GDP. In summary, we show that uncoupling protein 1 can affect reactive oxygen containing species production by isolated mitochondria from brown adipose tissue.

PMID:
20416274
DOI:
10.1016/j.bbabio.2010.04.008
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center